Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis. Using a...

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Detalles Bibliográficos
Autores principales: Tresenrider, Amy, Chia, Minghao, van Werven, Folkert J., Ünal, Elçin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829799/
https://www.ncbi.nlm.nih.gov/pubmed/35169715
http://dx.doi.org/10.1016/j.xpro.2022.101145