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Engineering and exploiting synthetic allostery of NanoLuc luciferase

Allostery enables proteins to interconvert different biochemical signals and form complex metabolic and signaling networks. We hypothesize that circular permutation of proteins increases the probability of functional coupling of new N- and C- termini with the protein’s active center through increase...

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Detalles Bibliográficos
Autores principales:	Guo, Zhong, Parakra, Rinky D., Xiong, Ying, Johnston, Wayne A., Walden, Patricia, Edwardraja, Selvakumar, Moradi, Shayli Varasteh, Ungerer, Jacobus P. J., Ai, Hui-wang, Phillips, Jonathan J., Alexandrov, Kirill
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831504/ https://www.ncbi.nlm.nih.gov/pubmed/35145068 http://dx.doi.org/10.1038/s41467-022-28425-2

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