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RT-qPCR Detection of SARS-CoV-2: No Need for a Dedicated Reverse Transcription Step

Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real-time, fluorescence-based usage (RT-qPCR), it has...

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Detalles Bibliográficos
Autores principales: Bustin, Stephen A., Shipley, Gregory L., Kirvell, Sara, Mueller, Reinhold, Nolan, Tania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835954/
https://www.ncbi.nlm.nih.gov/pubmed/35163227
http://dx.doi.org/10.3390/ijms23031303