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A Real-Time, Plate-Based BRET Assay for Detection of cGMP in Primary Cells

Cyclic guanosine monophosphate (cGMP) is a second messenger involved in the regulation of numerous physiological processes. The modulation of cGMP is important in many diseases, but reliably assaying cGMP in live cells in a plate-based format with temporal resolution is challenging. The Förster/fluo...

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Detalles Bibliográficos
Autores principales: Valkovic, Adam L., Kocan, Martina, Hoare, Brad, Marshall, Sarah, Scott, Daniel J., Bathgate, Ross A. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837005/
https://www.ncbi.nlm.nih.gov/pubmed/35163827
http://dx.doi.org/10.3390/ijms23031908