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Topoisomerase VI is a chirally-selective, preferential DNA decatenase
DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism for its preferential decatenation activity wa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837201/ https://www.ncbi.nlm.nih.gov/pubmed/35076393 http://dx.doi.org/10.7554/eLife.67021 |
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author | McKie, Shannon J Desai, Parth Rakesh Seol, Yeonee Allen, Adam MB Maxwell, Anthony Neuman, Keir C |
author_facet | McKie, Shannon J Desai, Parth Rakesh Seol, Yeonee Allen, Adam MB Maxwell, Anthony Neuman, Keir C |
author_sort | McKie, Shannon J |
collection | PubMed |
description | DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism for its preferential decatenation activity was lacking. Using single-molecule magnetic tweezers measurements and supporting ensemble biochemistry, we demonstrate that Methanosarcina mazei topo VI preferentially unlinks, or decatenates DNA crossings, in comparison to relaxing supercoils, through a preference for certain DNA crossing geometries. In addition, topo VI demonstrates a significant increase in ATPase activity, DNA binding and rate of strand passage, with increasing DNA writhe, providing further evidence that topo VI is a DNA crossing sensor. Our study strongly suggests that topo VI has evolved an intrinsic preference for the unknotting and decatenation of interlinked chromosomes by sensing and preferentially unlinking DNA crossings with geometries close to 90°. |
format | Online Article Text |
id | pubmed-8837201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-88372012022-02-14 Topoisomerase VI is a chirally-selective, preferential DNA decatenase McKie, Shannon J Desai, Parth Rakesh Seol, Yeonee Allen, Adam MB Maxwell, Anthony Neuman, Keir C eLife Biochemistry and Chemical Biology DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism for its preferential decatenation activity was lacking. Using single-molecule magnetic tweezers measurements and supporting ensemble biochemistry, we demonstrate that Methanosarcina mazei topo VI preferentially unlinks, or decatenates DNA crossings, in comparison to relaxing supercoils, through a preference for certain DNA crossing geometries. In addition, topo VI demonstrates a significant increase in ATPase activity, DNA binding and rate of strand passage, with increasing DNA writhe, providing further evidence that topo VI is a DNA crossing sensor. Our study strongly suggests that topo VI has evolved an intrinsic preference for the unknotting and decatenation of interlinked chromosomes by sensing and preferentially unlinking DNA crossings with geometries close to 90°. eLife Sciences Publications, Ltd 2022-01-25 /pmc/articles/PMC8837201/ /pubmed/35076393 http://dx.doi.org/10.7554/eLife.67021 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Biochemistry and Chemical Biology McKie, Shannon J Desai, Parth Rakesh Seol, Yeonee Allen, Adam MB Maxwell, Anthony Neuman, Keir C Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title | Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title_full | Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title_fullStr | Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title_full_unstemmed | Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title_short | Topoisomerase VI is a chirally-selective, preferential DNA decatenase |
title_sort | topoisomerase vi is a chirally-selective, preferential dna decatenase |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837201/ https://www.ncbi.nlm.nih.gov/pubmed/35076393 http://dx.doi.org/10.7554/eLife.67021 |
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