Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

The combination of ultrahigh-throughput screening and sequencing informs on function and intragenic epistasis within combinatorial protein mutant libraries. Establishing a droplet-based, in vitro compartmentalised approach for robust expression and screening of protein kinase cascades (>10(7) var...

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Detalles Bibliográficos
Autores principales: Scheele, Remkes A., Lindenburg, Laurens H., Petek, Maya, Schober, Markus, Dalby, Kevin N., Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837617/
https://www.ncbi.nlm.nih.gov/pubmed/35149678
http://dx.doi.org/10.1038/s41467-022-28396-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837617/
https://www.ncbi.nlm.nih.gov/pubmed/35149678
http://dx.doi.org/10.1038/s41467-022-28396-4

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