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Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?

Introduction: The skin prick test (SPT) is the first step in the diagnosis of an immunoglobulin E (IgE)-mediated food allergy. The availability of commercial food allergen extracts is very limited, resulting in a need for alternative extraction methods of food allergens. The objective of this study...

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Autores principales: Terlouw, Severina, van Boven, Frank E., Borsboom-van Zonneveld, Monika, de Graaf-in ‘t Veld, Catharina, van Splunter, Marloes E., van Daele, Paul L. A., van Maaren, Maurits S., Schreurs, Marco W. J., de Jong, Nicolette W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839075/
https://www.ncbi.nlm.nih.gov/pubmed/35276832
http://dx.doi.org/10.3390/nu14030475
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author Terlouw, Severina
van Boven, Frank E.
Borsboom-van Zonneveld, Monika
de Graaf-in ‘t Veld, Catharina
van Splunter, Marloes E.
van Daele, Paul L. A.
van Maaren, Maurits S.
Schreurs, Marco W. J.
de Jong, Nicolette W.
author_facet Terlouw, Severina
van Boven, Frank E.
Borsboom-van Zonneveld, Monika
de Graaf-in ‘t Veld, Catharina
van Splunter, Marloes E.
van Daele, Paul L. A.
van Maaren, Maurits S.
Schreurs, Marco W. J.
de Jong, Nicolette W.
author_sort Terlouw, Severina
collection PubMed
description Introduction: The skin prick test (SPT) is the first step in the diagnosis of an immunoglobulin E (IgE)-mediated food allergy. The availability of commercial food allergen extracts is very limited, resulting in a need for alternative extraction methods of food allergens. The objective of this study was to compare the SPT results of homemade food allergen extracts with commercially available extracts. Methods: Adult patients with a suspected food allergy were included. Food allergen-specific symptoms were scored using a questionnaire. SPTs were performed with homemade and commercially available extracts (ALK-Abelló, Kopenhagen, Denmark) from almond, apple, hazelnut, peach, peanut, and walnut. Serum-specific IgE was measured with ISAC or ImmunoCAP™. Intra-class correlation coefficients (ICC) between the SPT results of both extract methods were calculated. The proportion of agreement with food allergen-specific symptoms was analyzed. Results: Fifty-four patients (mean age 36; range 19–69 years; female/male: 42/12) were included. The intra-class correlation coefficient (ICC) between the SPT results of both extract methods were strong for hazelnut 0.79 (n = 44) and walnut 0.78 (n = 31), moderate for apple 0.74 (n = 21) and peanut 0.66 (n = 28), and weak for almond 0.36 (n = 27) and peach 0.17 (n = 23). The proportion of agreement between SPT results and food allergen-specific symptoms was comparable for homemade and commercially available extracts, except for peach; 0.77 versus 0.36, respectively. Conclusion: In the diagnostic procedures to identify an IgE-mediated food allergy, homemade extracts from hazelnut and walnut appear to be a good alternative in the absence of commercially available food allergen extracts.
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spelling pubmed-88390752022-02-13 Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts? Terlouw, Severina van Boven, Frank E. Borsboom-van Zonneveld, Monika de Graaf-in ‘t Veld, Catharina van Splunter, Marloes E. van Daele, Paul L. A. van Maaren, Maurits S. Schreurs, Marco W. J. de Jong, Nicolette W. Nutrients Article Introduction: The skin prick test (SPT) is the first step in the diagnosis of an immunoglobulin E (IgE)-mediated food allergy. The availability of commercial food allergen extracts is very limited, resulting in a need for alternative extraction methods of food allergens. The objective of this study was to compare the SPT results of homemade food allergen extracts with commercially available extracts. Methods: Adult patients with a suspected food allergy were included. Food allergen-specific symptoms were scored using a questionnaire. SPTs were performed with homemade and commercially available extracts (ALK-Abelló, Kopenhagen, Denmark) from almond, apple, hazelnut, peach, peanut, and walnut. Serum-specific IgE was measured with ISAC or ImmunoCAP™. Intra-class correlation coefficients (ICC) between the SPT results of both extract methods were calculated. The proportion of agreement with food allergen-specific symptoms was analyzed. Results: Fifty-four patients (mean age 36; range 19–69 years; female/male: 42/12) were included. The intra-class correlation coefficient (ICC) between the SPT results of both extract methods were strong for hazelnut 0.79 (n = 44) and walnut 0.78 (n = 31), moderate for apple 0.74 (n = 21) and peanut 0.66 (n = 28), and weak for almond 0.36 (n = 27) and peach 0.17 (n = 23). The proportion of agreement between SPT results and food allergen-specific symptoms was comparable for homemade and commercially available extracts, except for peach; 0.77 versus 0.36, respectively. Conclusion: In the diagnostic procedures to identify an IgE-mediated food allergy, homemade extracts from hazelnut and walnut appear to be a good alternative in the absence of commercially available food allergen extracts. MDPI 2022-01-21 /pmc/articles/PMC8839075/ /pubmed/35276832 http://dx.doi.org/10.3390/nu14030475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Terlouw, Severina
van Boven, Frank E.
Borsboom-van Zonneveld, Monika
de Graaf-in ‘t Veld, Catharina
van Splunter, Marloes E.
van Daele, Paul L. A.
van Maaren, Maurits S.
Schreurs, Marco W. J.
de Jong, Nicolette W.
Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title_full Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title_fullStr Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title_full_unstemmed Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title_short Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially Available Extracts?
title_sort homemade food allergen extracts for use in skin prick tests in the diagnosis of ige-mediated food allergy: a good alternative in the absence of commercially available extracts?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839075/
https://www.ncbi.nlm.nih.gov/pubmed/35276832
http://dx.doi.org/10.3390/nu14030475
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