Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and off-target effects. CRISPR delivered as ribonuc...

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Detalles Bibliográficos
Autores principales: Zhang, Yingxiao, Cheng, Yanhao, Fang, Hong, Roberts, Nathaniel, Zhang, Liyang, Vakulskas, Christopher A., Niedz, Randall P., Culver, James N., Qi, Yiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8842731/
https://www.ncbi.nlm.nih.gov/pubmed/35174354
http://dx.doi.org/10.3389/fgeed.2022.780238

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8842731/
https://www.ncbi.nlm.nih.gov/pubmed/35174354
http://dx.doi.org/10.3389/fgeed.2022.780238

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