dCas9-based gene editing for cleavage-free genomic knock-in of long sequences

Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene editing could cause DNA damage and trigger DNA repair processes that are often error-prone. Such unwanted mutations and safety concerns can be exacerbated when altering long sequences. Here we couple mic...

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Detalles Bibliográficos
Autores principales: Wang, Chengkun, Qu, Yuanhao, Cheng, Jason K. W., Hughes, Nicholas W., Zhang, Qianhe, Wang, Mengdi, Cong, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843813/
https://www.ncbi.nlm.nih.gov/pubmed/35145221
http://dx.doi.org/10.1038/s41556-021-00836-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843813/
https://www.ncbi.nlm.nih.gov/pubmed/35145221
http://dx.doi.org/10.1038/s41556-021-00836-1

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