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Isolation and feeder-free primary culture of four cell types from a single human skin sample

Four types of primary cells—dermal fibroblasts, dermal microvascular endothelial cells, epidermal keratinocytes, and epidermal melanocytes—can be isolated simultaneously from a single human skin sample, without the use of xenogeneic murine feeder cells. This protocol describes the procedures for iso...

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Detalles Bibliográficos
Autores principales: Supp, Dorothy M., Hahn, Jennifer M., Combs, Kelly A., McFarland, Kevin L., Powell, Heather M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844903/
https://www.ncbi.nlm.nih.gov/pubmed/35199036
http://dx.doi.org/10.1016/j.xpro.2022.101172