2′-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR–Cas12a systems

The CRISPR–Cas12a system has been widely applied to genome editing and molecular diagnostics. However, off-target cleavages and false-positive results remain as major concerns in Cas12a practical applications. Herein, we propose a strategy by utilizing the 2′-O-methyl (2′-OMe) modified guide RNA (gR...

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Detalles Bibliográficos
Autores principales: Ke, Yuqing, Ghalandari, Behafarid, Huang, Shiyi, Li, Sijie, Huang, Chengjie, Zhi, Xiao, Cui, Daxiang, Ding, Xianting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848812/
https://www.ncbi.nlm.nih.gov/pubmed/35308857
http://dx.doi.org/10.1039/d1sc06832f

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848812/
https://www.ncbi.nlm.nih.gov/pubmed/35308857
http://dx.doi.org/10.1039/d1sc06832f

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