PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing

The CRISPR-Cas system has been repurposed as a powerful live-cell imaging tool, but its utility is limited to genomic loci and mRNA imaging in living cells. Here, we demonstrated the potential of the CRISPR-Cas system as a generalizable live-cell biosensing tool by extending its applicability to mon...

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Detalles Bibliográficos
Autores principales: Chen, Siyu, Wang, Rujia, Peng, Shuang, Xie, Shiyi, Lei, Chunyang, Huang, Yan, Nie, Zhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848855/
https://www.ncbi.nlm.nih.gov/pubmed/35308851
http://dx.doi.org/10.1039/d1sc05558e