Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica

Genome-wide functional genetic screens have been successful in discovering genotype-phenotype relationships and in engineering new phenotypes. While broadly applied in mammalian cell lines and in E. coli, use in non-conventional microorganisms has been limited, in part, due to the inability to accur...

Descripción completa

Detalles Bibliográficos
Autores principales: Baisya, Dipankar, Ramesh, Adithya, Schwartz, Cory, Lonardi, Stefano, Wheeldon, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854577/
https://www.ncbi.nlm.nih.gov/pubmed/35177617
http://dx.doi.org/10.1038/s41467-022-28540-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854577/
https://www.ncbi.nlm.nih.gov/pubmed/35177617
http://dx.doi.org/10.1038/s41467-022-28540-0

Ejemplares similares