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Generation of a pooled shRNA library for functional genomics screens

Here, we detail a protocol for the generation of pooled short hairpin RNA (shRNA) libraries. We cover the design of optimized miR-E backbone shRNAs, cloning into a Tet-on vector system, and transformation of competent bacteria. We also describe library quality check by next-generation sequencing, an...

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Detalles Bibliográficos
Autores principales: Papadopoulos, Dimitrios, Ade, Carsten Patrick, Eilers, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857607/
https://www.ncbi.nlm.nih.gov/pubmed/35243374
http://dx.doi.org/10.1016/j.xpro.2022.101183