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Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas
Carboxypeptidase A1 (CPA1) is a zinc metalloprotease that is produced in pancreatic acinar cells and plays a role in cleaving C-terminal branched-chain and aromatic amino acids from dietary proteins. This study assessed the utility of immunohistochemical CPA1 staining for diagnosing pancreatic acina...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860221/ https://www.ncbi.nlm.nih.gov/pubmed/34889867 http://dx.doi.org/10.1097/PAS.0000000000001817 |
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author | Uhlig, Ria Contreras, Hendrina Weidemann, Sören Gorbokon, Natalia Menz, Anne Büscheck, Franziska Luebke, Andreas M. Kluth, Martina Hube-Magg, Claudia Hinsch, Andrea Höflmayer, Doris Fraune, Christoph Möller, Katharina Bernreuther, Christian Lebok, Patrick Sauter, Guido Wilczak, Waldemar Izbicki, Jakob Perez, Daniel Schrader, Jörg Steurer, Stefan Burandt, Eike Krech, Rainer Dum, David Krech, Till Marx, Andreas Simon, Ronald Minner, Sarah Jacobsen, Frank Clauditz, Till S. |
author_facet | Uhlig, Ria Contreras, Hendrina Weidemann, Sören Gorbokon, Natalia Menz, Anne Büscheck, Franziska Luebke, Andreas M. Kluth, Martina Hube-Magg, Claudia Hinsch, Andrea Höflmayer, Doris Fraune, Christoph Möller, Katharina Bernreuther, Christian Lebok, Patrick Sauter, Guido Wilczak, Waldemar Izbicki, Jakob Perez, Daniel Schrader, Jörg Steurer, Stefan Burandt, Eike Krech, Rainer Dum, David Krech, Till Marx, Andreas Simon, Ronald Minner, Sarah Jacobsen, Frank Clauditz, Till S. |
author_sort | Uhlig, Ria |
collection | PubMed |
description | Carboxypeptidase A1 (CPA1) is a zinc metalloprotease that is produced in pancreatic acinar cells and plays a role in cleaving C-terminal branched-chain and aromatic amino acids from dietary proteins. This study assessed the utility of immunohistochemical CPA1 staining for diagnosing pancreatic acinar cell carcinoma (ACC). A total of 12,274 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types were interpretable by immunohistochemistry in a tissue microarray format. CPA1 was strongly expressed in acinar cells of all normal pancreas samples but not in any other normal tissues. CPA1 immunostaining was detected in 100% of 11 pancreatic ACCs and 1 mixed acinar endocrine carcinoma, but absent in 449 pancreatic ductal adenocarcinomas, 75 adenocarcinomas of the ampulla Vateri, and 11,739 other evaluable cancers from 128 different tumor entities. A weak to moderate diffuse staining of epithelial and stromal cells of cancer tissues immediately adjacent to non-neoplastic pancreatic acinar cells often occurred and was considered to be caused by the diffusion of the highly abundant CPA1 from normal acinar cells that may have suffered some autolytic cell damage. In conclusion, our data show that CPA1 is a highly sensitive and largely specific marker for normal and neoplastic pancreatic acinar cells. CPA1 immunohistochemistry greatly facilitates the otherwise often difficult diagnosis of pancreatic ACC. |
format | Online Article Text |
id | pubmed-8860221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-88602212022-02-24 Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas Uhlig, Ria Contreras, Hendrina Weidemann, Sören Gorbokon, Natalia Menz, Anne Büscheck, Franziska Luebke, Andreas M. Kluth, Martina Hube-Magg, Claudia Hinsch, Andrea Höflmayer, Doris Fraune, Christoph Möller, Katharina Bernreuther, Christian Lebok, Patrick Sauter, Guido Wilczak, Waldemar Izbicki, Jakob Perez, Daniel Schrader, Jörg Steurer, Stefan Burandt, Eike Krech, Rainer Dum, David Krech, Till Marx, Andreas Simon, Ronald Minner, Sarah Jacobsen, Frank Clauditz, Till S. Am J Surg Pathol Original Articles Carboxypeptidase A1 (CPA1) is a zinc metalloprotease that is produced in pancreatic acinar cells and plays a role in cleaving C-terminal branched-chain and aromatic amino acids from dietary proteins. This study assessed the utility of immunohistochemical CPA1 staining for diagnosing pancreatic acinar cell carcinoma (ACC). A total of 12,274 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types were interpretable by immunohistochemistry in a tissue microarray format. CPA1 was strongly expressed in acinar cells of all normal pancreas samples but not in any other normal tissues. CPA1 immunostaining was detected in 100% of 11 pancreatic ACCs and 1 mixed acinar endocrine carcinoma, but absent in 449 pancreatic ductal adenocarcinomas, 75 adenocarcinomas of the ampulla Vateri, and 11,739 other evaluable cancers from 128 different tumor entities. A weak to moderate diffuse staining of epithelial and stromal cells of cancer tissues immediately adjacent to non-neoplastic pancreatic acinar cells often occurred and was considered to be caused by the diffusion of the highly abundant CPA1 from normal acinar cells that may have suffered some autolytic cell damage. In conclusion, our data show that CPA1 is a highly sensitive and largely specific marker for normal and neoplastic pancreatic acinar cells. CPA1 immunohistochemistry greatly facilitates the otherwise often difficult diagnosis of pancreatic ACC. Lippincott Williams & Wilkins 2022-01 2021-10-21 /pmc/articles/PMC8860221/ /pubmed/34889867 http://dx.doi.org/10.1097/PAS.0000000000001817 Text en Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Original Articles Uhlig, Ria Contreras, Hendrina Weidemann, Sören Gorbokon, Natalia Menz, Anne Büscheck, Franziska Luebke, Andreas M. Kluth, Martina Hube-Magg, Claudia Hinsch, Andrea Höflmayer, Doris Fraune, Christoph Möller, Katharina Bernreuther, Christian Lebok, Patrick Sauter, Guido Wilczak, Waldemar Izbicki, Jakob Perez, Daniel Schrader, Jörg Steurer, Stefan Burandt, Eike Krech, Rainer Dum, David Krech, Till Marx, Andreas Simon, Ronald Minner, Sarah Jacobsen, Frank Clauditz, Till S. Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title | Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title_full | Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title_fullStr | Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title_full_unstemmed | Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title_short | Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas |
title_sort | carboxypeptidase a1 (cpa1) immunohistochemistry is highly sensitive and specific for acinar cell carcinoma (acc) of the pancreas |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860221/ https://www.ncbi.nlm.nih.gov/pubmed/34889867 http://dx.doi.org/10.1097/PAS.0000000000001817 |
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