High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination

The Cas9 nuclease from Staphylococcus aureus (SaCas9) holds great potential for use in gene therapy, and variants with increased fidelity have been engineered. However, we find that existing variants have not reached the greatest accuracy to discriminate base mismatches and exhibited much reduced ac...

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Detalles Bibliográficos
Autores principales: Yuen, Chaya T L, Thean, Dawn G L, Chan, Becky K C, Zhou, Peng, Kwok, Cynthia C S, Chu, Hoi Yee, Cheung, Maggie S H, Wang, Bei, Chan, Yee Man, Mak, Silvia Y L, Leung, Anskar Y, Choi, Gigi C G, Zheng, Zongli, Wong, Alan S L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860571/
https://www.ncbi.nlm.nih.gov/pubmed/35051997
http://dx.doi.org/10.1093/nar/gkab1291

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860571/
https://www.ncbi.nlm.nih.gov/pubmed/35051997
http://dx.doi.org/10.1093/nar/gkab1291

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