Cargando…
High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
The Cas9 nuclease from Staphylococcus aureus (SaCas9) holds great potential for use in gene therapy, and variants with increased fidelity have been engineered. However, we find that existing variants have not reached the greatest accuracy to discriminate base mismatches and exhibited much reduced ac...
Autores principales: | Yuen, Chaya T L, Thean, Dawn G L, Chan, Becky K C, Zhou, Peng, Kwok, Cynthia C S, Chu, Hoi Yee, Cheung, Maggie S H, Wang, Bei, Chan, Yee Man, Mak, Silvia Y L, Leung, Anskar Y, Choi, Gigi C G, Zheng, Zongli, Wong, Alan S L |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860571/ https://www.ncbi.nlm.nih.gov/pubmed/35051997 http://dx.doi.org/10.1093/nar/gkab1291 |
Ejemplares similares
-
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
por: Tóth, Eszter, et al.
Publicado: (2018) -
Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
por: Fu, Becky X.H., et al.
Publicado: (2016) -
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion
por: Şöllü, Cem, et al.
Publicado: (2010) -
A novel DNA nuclease is stimulated by association with the GINS complex
por: Li, Zhuo, et al.
Publicado: (2011) -
Dna2 is a structure-specific nuclease, with affinity for 5′-flap intermediates
por: Stewart, Jason A., et al.
Publicado: (2010)