Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria

Recombineering assisted multiplex genome editing generally uses single-stranded oligonucleotides for site directed mutational changes. It has proven highly efficient for functional screens and to optimize microbial cell factories. However, this approach is limited to relatively small mutational chan...

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Detalles Bibliográficos
Autores principales: Wang, Xue, Zheng, Wentao, Zhou, Haibo, Tu, Qiang, Tang, Ya-Jie, Stewart, A Francis, Zhang, Youming, Bian, Xiaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860599/
https://www.ncbi.nlm.nih.gov/pubmed/34792175
http://dx.doi.org/10.1093/nar/gkab1076

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860599/
https://www.ncbi.nlm.nih.gov/pubmed/34792175
http://dx.doi.org/10.1093/nar/gkab1076

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