Cargando…
Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability
Methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) are zoonotic life-threatening pathogens, and their presence in food raises a public health concern. Yet, scarce data are available regarding MRSA and VRSA in both ready-to-eat (RTE) meat and food handlers. This stud...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861318/ https://www.ncbi.nlm.nih.gov/pubmed/35211098 http://dx.doi.org/10.3389/fmicb.2021.735494 |
_version_ | 1784654863025569792 |
---|---|
author | Saber, Taisir Samir, Mohamed El-Mekkawy, Rasha M. Ariny, Eman El-Sayed, Sara Ramadan Enan, Gamal Abdelatif, Sawasn H. Askora, Ahmed Merwad, Abdallah M. A. Tartor, Yasmine H. |
author_facet | Saber, Taisir Samir, Mohamed El-Mekkawy, Rasha M. Ariny, Eman El-Sayed, Sara Ramadan Enan, Gamal Abdelatif, Sawasn H. Askora, Ahmed Merwad, Abdallah M. A. Tartor, Yasmine H. |
author_sort | Saber, Taisir |
collection | PubMed |
description | Methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) are zoonotic life-threatening pathogens, and their presence in food raises a public health concern. Yet, scarce data are available regarding MRSA and VRSA in both ready-to-eat (RTE) meat and food handlers. This study was undertaken to determine the frequency, antimicrobial resistance, and biofilm-forming ability of MRSA and VRSA isolated from RTE meat (shawarma and burger) and humans (food handlers, and hospitalized patients) in Zagazig city, Sharkia Governorate, Egypt. We analyzed 176 samples (112 human samples: 72 from hospitalized patients and 40 from food handlers, 64 RTE meat samples: 38 from shawarma and 26 from burger). Using phenotypic, PCR-based identification of nuc gene and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), 60 coagulase-positive S. aureus (COPS) isolates were identified in the samples as follow: RTE meat (15/64, 23.4%), hospitalized patients (33/72, 45.8%) and food handlers (12/40, 30%). All the COPS isolates were mecA positive (and thus were classified as MRSA) and multidrug resistant with multiple antibiotic resistance indices ranging from 0.25 to 0.92. Overall, resistance to cefepime (96.7%), penicillin (88.3%), were common, followed by ampicillin-sulbactam (65%), ciprofloxacin (55%), nitrofurontoin (51.7%), and gentamicin (43.3%). VRSA was detected in 30.3% of COPS hospitalized patient’s isolates, 26.7% of COPS RTE meat isolates and 25% of COPS food handler’s isolates. VanA, vanB, or both genes were detected in 64.7, 5.9, and 29.4% of all VAN-resistant isolates, respectively. The majority of the COPS isolates (50/60, 83.3%) have biofilm formation ability and harbored icaA (76%), icaD (74%), icaC (50%), and icaB (46%) biofilm-forming genes. The bap gene was not detected in any of the isolates. The ability of MRSA and VRSA isolates to produce biofilms in addition to being resistant to antimicrobials highlight the danger posed by these potentially virulent microorganisms persisting in RTE meat, food handlers, and patients. Taken together, good hygiene practices and antimicrobial surveillance plans should be strictly implemented along the food chain to reduce the risk of colonization and dissemination of MRSA and VRSA biofilm-producing strains. |
format | Online Article Text |
id | pubmed-8861318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88613182022-02-23 Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability Saber, Taisir Samir, Mohamed El-Mekkawy, Rasha M. Ariny, Eman El-Sayed, Sara Ramadan Enan, Gamal Abdelatif, Sawasn H. Askora, Ahmed Merwad, Abdallah M. A. Tartor, Yasmine H. Front Microbiol Microbiology Methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) are zoonotic life-threatening pathogens, and their presence in food raises a public health concern. Yet, scarce data are available regarding MRSA and VRSA in both ready-to-eat (RTE) meat and food handlers. This study was undertaken to determine the frequency, antimicrobial resistance, and biofilm-forming ability of MRSA and VRSA isolated from RTE meat (shawarma and burger) and humans (food handlers, and hospitalized patients) in Zagazig city, Sharkia Governorate, Egypt. We analyzed 176 samples (112 human samples: 72 from hospitalized patients and 40 from food handlers, 64 RTE meat samples: 38 from shawarma and 26 from burger). Using phenotypic, PCR-based identification of nuc gene and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), 60 coagulase-positive S. aureus (COPS) isolates were identified in the samples as follow: RTE meat (15/64, 23.4%), hospitalized patients (33/72, 45.8%) and food handlers (12/40, 30%). All the COPS isolates were mecA positive (and thus were classified as MRSA) and multidrug resistant with multiple antibiotic resistance indices ranging from 0.25 to 0.92. Overall, resistance to cefepime (96.7%), penicillin (88.3%), were common, followed by ampicillin-sulbactam (65%), ciprofloxacin (55%), nitrofurontoin (51.7%), and gentamicin (43.3%). VRSA was detected in 30.3% of COPS hospitalized patient’s isolates, 26.7% of COPS RTE meat isolates and 25% of COPS food handler’s isolates. VanA, vanB, or both genes were detected in 64.7, 5.9, and 29.4% of all VAN-resistant isolates, respectively. The majority of the COPS isolates (50/60, 83.3%) have biofilm formation ability and harbored icaA (76%), icaD (74%), icaC (50%), and icaB (46%) biofilm-forming genes. The bap gene was not detected in any of the isolates. The ability of MRSA and VRSA isolates to produce biofilms in addition to being resistant to antimicrobials highlight the danger posed by these potentially virulent microorganisms persisting in RTE meat, food handlers, and patients. Taken together, good hygiene practices and antimicrobial surveillance plans should be strictly implemented along the food chain to reduce the risk of colonization and dissemination of MRSA and VRSA biofilm-producing strains. Frontiers Media S.A. 2022-02-08 /pmc/articles/PMC8861318/ /pubmed/35211098 http://dx.doi.org/10.3389/fmicb.2021.735494 Text en Copyright © 2022 Saber, Samir, El-Mekkawy, Ariny, El-Sayed, Enan, Abdelatif, Askora, Merwad and Tartor. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Saber, Taisir Samir, Mohamed El-Mekkawy, Rasha M. Ariny, Eman El-Sayed, Sara Ramadan Enan, Gamal Abdelatif, Sawasn H. Askora, Ahmed Merwad, Abdallah M. A. Tartor, Yasmine H. Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title | Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title_full | Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title_fullStr | Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title_full_unstemmed | Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title_short | Methicillin- and Vancomycin-Resistant Staphylococcus aureus From Humans and Ready-To-Eat Meat: Characterization of Antimicrobial Resistance and Biofilm Formation Ability |
title_sort | methicillin- and vancomycin-resistant staphylococcus aureus from humans and ready-to-eat meat: characterization of antimicrobial resistance and biofilm formation ability |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861318/ https://www.ncbi.nlm.nih.gov/pubmed/35211098 http://dx.doi.org/10.3389/fmicb.2021.735494 |
work_keys_str_mv | AT sabertaisir methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT samirmohamed methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT elmekkawyrasham methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT arinyeman methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT elsayedsararamadan methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT enangamal methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT abdelatifsawasnh methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT askoraahmed methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT merwadabdallahma methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability AT tartoryasmineh methicillinandvancomycinresistantstaphylococcusaureusfromhumansandreadytoeatmeatcharacterizationofantimicrobialresistanceandbiofilmformationability |