Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China

The Qinghai–Tibet Plateau Area (QTPA) has a complex natural ecosystem, causing a greatly increased risk of spreading various tick-borne diseases including rickettsial infections, which are regarded as one of the oldest known vector-borne zoonoses. However, the information of one of its pathogen, spo...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Yong-Cai, Li, Ji-Xu, Sun, Ya-Li, Kang, Ming, He, Hong-Xuan, Guo, Yun-Hai, Ma, Ping, Wei, Yao-Ping, Li, Rui-Shan, Chen, Wang-Kai, Chen, Zhi-Hong, Li, Jing, Qi, Tong-Sheng, Yang, Jin-Fang, Zhang, Qing-Xun, Wang, Ye, Cai, Jin-Shan, Zhao, Quan-Bang, Hu, Guang-Wei, Chen, Ji-Yong, Li, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861378/
https://www.ncbi.nlm.nih.gov/pubmed/35211533
http://dx.doi.org/10.3389/fvets.2021.779387
_version_ 1784654871773839360
author He, Yong-Cai
Li, Ji-Xu
Sun, Ya-Li
Kang, Ming
He, Hong-Xuan
Guo, Yun-Hai
Ma, Ping
Wei, Yao-Ping
Li, Rui-Shan
Chen, Wang-Kai
Chen, Zhi-Hong
Li, Jing
Qi, Tong-Sheng
Yang, Jin-Fang
Zhang, Qing-Xun
Wang, Ye
Cai, Jin-Shan
Zhao, Quan-Bang
Hu, Guang-Wei
Chen, Ji-Yong
Li, Ying
author_facet He, Yong-Cai
Li, Ji-Xu
Sun, Ya-Li
Kang, Ming
He, Hong-Xuan
Guo, Yun-Hai
Ma, Ping
Wei, Yao-Ping
Li, Rui-Shan
Chen, Wang-Kai
Chen, Zhi-Hong
Li, Jing
Qi, Tong-Sheng
Yang, Jin-Fang
Zhang, Qing-Xun
Wang, Ye
Cai, Jin-Shan
Zhao, Quan-Bang
Hu, Guang-Wei
Chen, Ji-Yong
Li, Ying
author_sort He, Yong-Cai
collection PubMed
description The Qinghai–Tibet Plateau Area (QTPA) has a complex natural ecosystem, causing a greatly increased risk of spreading various tick-borne diseases including rickettsial infections, which are regarded as one of the oldest known vector-borne zoonoses. However, the information of one of its pathogen, spotted fever group Rickettsia (SFG Rickettsia), is limited in tick vectors and animals in this area. Therefore, this study focused on the investigation of SFG Rickettsia in tick vectors, yaks (Bos grunniens), and Tibetan sheep (Ovis aries) in the QTPA. A total of 1,000 samples were collected from nine sampling sites, including 425 of yaks, 309 of Tibetan sheep, 266 of ticks. By morphological examination, PCR, and sequencing, we confirmed the species of all collected ticks. All tick samples, all yak and Tibetan sheep blood samples were detected based on SFG Rickettsia ompA and sca4 gene. The results showed that all tick samples were identified to be Haemaphysalis qinghaiensis, and the positive rates of SFG Rickettsia were 5.9% (25/425), 0.3% (1/309), and 54.1% (144/266) in yaks, Tibetan sheep, and ticks, respectively. All positive samples were sequenced, and BLASTn analysis of the ompA gene sequences of SFG Rickettsia showed that all positive samples from animals and ticks had 99.04–100% identity with yak and horse isolates from Qinghai Province, China. BLASTn analysis of the sca4 gene sequences of SFG Rickettsia showed that all positive samples had 97.60–98.72% identity with tick isolates from Ukraine. In addition, the phylogenetic analysis showed that all the SFG Rickettsia ompA and sca4 sequences obtained from this study belong to the same clade as Rickettsia raoultii isolated from livestock and ticks from China and other countries. Molecularly, this study detected and characterized SFG Rickettsia both in the tick vectors and animals, suggesting that the relationship between SFG Rickettsia, tick species and animal hosts should be explored to understand their interrelationships, which provide a theoretical basis for preventing control of this pathogen.
format Online
Article
Text
id pubmed-8861378
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88613782022-02-23 Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China He, Yong-Cai Li, Ji-Xu Sun, Ya-Li Kang, Ming He, Hong-Xuan Guo, Yun-Hai Ma, Ping Wei, Yao-Ping Li, Rui-Shan Chen, Wang-Kai Chen, Zhi-Hong Li, Jing Qi, Tong-Sheng Yang, Jin-Fang Zhang, Qing-Xun Wang, Ye Cai, Jin-Shan Zhao, Quan-Bang Hu, Guang-Wei Chen, Ji-Yong Li, Ying Front Vet Sci Veterinary Science The Qinghai–Tibet Plateau Area (QTPA) has a complex natural ecosystem, causing a greatly increased risk of spreading various tick-borne diseases including rickettsial infections, which are regarded as one of the oldest known vector-borne zoonoses. However, the information of one of its pathogen, spotted fever group Rickettsia (SFG Rickettsia), is limited in tick vectors and animals in this area. Therefore, this study focused on the investigation of SFG Rickettsia in tick vectors, yaks (Bos grunniens), and Tibetan sheep (Ovis aries) in the QTPA. A total of 1,000 samples were collected from nine sampling sites, including 425 of yaks, 309 of Tibetan sheep, 266 of ticks. By morphological examination, PCR, and sequencing, we confirmed the species of all collected ticks. All tick samples, all yak and Tibetan sheep blood samples were detected based on SFG Rickettsia ompA and sca4 gene. The results showed that all tick samples were identified to be Haemaphysalis qinghaiensis, and the positive rates of SFG Rickettsia were 5.9% (25/425), 0.3% (1/309), and 54.1% (144/266) in yaks, Tibetan sheep, and ticks, respectively. All positive samples were sequenced, and BLASTn analysis of the ompA gene sequences of SFG Rickettsia showed that all positive samples from animals and ticks had 99.04–100% identity with yak and horse isolates from Qinghai Province, China. BLASTn analysis of the sca4 gene sequences of SFG Rickettsia showed that all positive samples had 97.60–98.72% identity with tick isolates from Ukraine. In addition, the phylogenetic analysis showed that all the SFG Rickettsia ompA and sca4 sequences obtained from this study belong to the same clade as Rickettsia raoultii isolated from livestock and ticks from China and other countries. Molecularly, this study detected and characterized SFG Rickettsia both in the tick vectors and animals, suggesting that the relationship between SFG Rickettsia, tick species and animal hosts should be explored to understand their interrelationships, which provide a theoretical basis for preventing control of this pathogen. Frontiers Media S.A. 2022-02-08 /pmc/articles/PMC8861378/ /pubmed/35211533 http://dx.doi.org/10.3389/fvets.2021.779387 Text en Copyright © 2022 He, Li, Sun, Kang, He, Guo, Ma, Wei, Li, Chen, Chen, Li, Qi, Yang, Zhang, Wang, Cai, Zhao, Hu, Chen and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
He, Yong-Cai
Li, Ji-Xu
Sun, Ya-Li
Kang, Ming
He, Hong-Xuan
Guo, Yun-Hai
Ma, Ping
Wei, Yao-Ping
Li, Rui-Shan
Chen, Wang-Kai
Chen, Zhi-Hong
Li, Jing
Qi, Tong-Sheng
Yang, Jin-Fang
Zhang, Qing-Xun
Wang, Ye
Cai, Jin-Shan
Zhao, Quan-Bang
Hu, Guang-Wei
Chen, Ji-Yong
Li, Ying
Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title_full Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title_fullStr Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title_full_unstemmed Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title_short Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
title_sort spotted fever group rickettsia infecting ticks (acari: ixodidae), yak (bos grunniens), and tibetan sheep (ovis aries) in the qinghai–tibetan plateau area, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861378/
https://www.ncbi.nlm.nih.gov/pubmed/35211533
http://dx.doi.org/10.3389/fvets.2021.779387
work_keys_str_mv AT heyongcai spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT lijixu spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT sunyali spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT kangming spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT hehongxuan spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT guoyunhai spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT maping spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT weiyaoping spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT liruishan spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT chenwangkai spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT chenzhihong spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT lijing spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT qitongsheng spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT yangjinfang spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT zhangqingxun spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT wangye spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT caijinshan spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT zhaoquanbang spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT huguangwei spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT chenjiyong spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina
AT liying spottedfevergrouprickettsiainfectingticksacariixodidaeyakbosgrunniensandtibetansheepovisariesintheqinghaitibetanplateauareachina