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Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units

Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular vesicles (EVs) throughout their lifespan in the circulation, and especially during senescence, by spleen-facilitated vesiculation of their membrane. During ex vivo aging under blood bank conditions, the RBCs lose Hb, both in sol...

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Autores principales: Tzounakas, Vassilis L., Anastasiadi, Alkmini T., Lekka, Marilena E., Papageorgiou, Effie G., Stamoulis, Konstantinos, Papassideri, Issidora S., Kriebardis, Anastasios G., Antonelou, Marianna H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861500/
https://www.ncbi.nlm.nih.gov/pubmed/35211035
http://dx.doi.org/10.3389/fphys.2022.840995
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author Tzounakas, Vassilis L.
Anastasiadi, Alkmini T.
Lekka, Marilena E.
Papageorgiou, Effie G.
Stamoulis, Konstantinos
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
author_facet Tzounakas, Vassilis L.
Anastasiadi, Alkmini T.
Lekka, Marilena E.
Papageorgiou, Effie G.
Stamoulis, Konstantinos
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
author_sort Tzounakas, Vassilis L.
collection PubMed
description Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular vesicles (EVs) throughout their lifespan in the circulation, and especially during senescence, by spleen-facilitated vesiculation of their membrane. During ex vivo aging under blood bank conditions, the RBCs lose Hb, both in soluble form and inside EVs that accumulate as a part of storage lesion in the supernatant of the unit. Spontaneous hemolysis and vesiculation are increasingly promoted by the storage duration, but little is known about any physiological linkage between them. In the present study, we measured the levels of total extracellular and EV-enclosed Hb (EV-Hb) in units of whole blood (n = 36) or packed RBCs stored in either CPDA-1 (n = 99) or in CPD-SAGM additive solution (n = 46), in early, middle, and late storage. The spectrophotometry data were subjected to statistical analysis to detect possible correlation(s) between storage hemolysis and EV-Hb, as well as the threshold (if any) that determines the area of this dynamic association. It seems that the percentage of EV-Hb is negatively associated with hemolysis levels from middle storage onward by showing low to moderate correlation profiles in all strategies under investigation. Moreover, 0.17% storage hemolysis was determined as the potential cut-off, above which this inverse correlation is evident in non-leukoreduced CPDA units. Notably, RBC units with hemolysis levels > 0.17% are characterized by higher percentage of nanovesicles (<100 nm) over typical microvesicles (100–400 nm) compared with the lower hemolysis counterparts. Our results suggest an ordered loss of Hb during RBC accelerated aging that might fuel targeted research to elucidate its mechanistic basis.
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spelling pubmed-88615002022-02-23 Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units Tzounakas, Vassilis L. Anastasiadi, Alkmini T. Lekka, Marilena E. Papageorgiou, Effie G. Stamoulis, Konstantinos Papassideri, Issidora S. Kriebardis, Anastasios G. Antonelou, Marianna H. Front Physiol Physiology Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular vesicles (EVs) throughout their lifespan in the circulation, and especially during senescence, by spleen-facilitated vesiculation of their membrane. During ex vivo aging under blood bank conditions, the RBCs lose Hb, both in soluble form and inside EVs that accumulate as a part of storage lesion in the supernatant of the unit. Spontaneous hemolysis and vesiculation are increasingly promoted by the storage duration, but little is known about any physiological linkage between them. In the present study, we measured the levels of total extracellular and EV-enclosed Hb (EV-Hb) in units of whole blood (n = 36) or packed RBCs stored in either CPDA-1 (n = 99) or in CPD-SAGM additive solution (n = 46), in early, middle, and late storage. The spectrophotometry data were subjected to statistical analysis to detect possible correlation(s) between storage hemolysis and EV-Hb, as well as the threshold (if any) that determines the area of this dynamic association. It seems that the percentage of EV-Hb is negatively associated with hemolysis levels from middle storage onward by showing low to moderate correlation profiles in all strategies under investigation. Moreover, 0.17% storage hemolysis was determined as the potential cut-off, above which this inverse correlation is evident in non-leukoreduced CPDA units. Notably, RBC units with hemolysis levels > 0.17% are characterized by higher percentage of nanovesicles (<100 nm) over typical microvesicles (100–400 nm) compared with the lower hemolysis counterparts. Our results suggest an ordered loss of Hb during RBC accelerated aging that might fuel targeted research to elucidate its mechanistic basis. Frontiers Media S.A. 2022-02-08 /pmc/articles/PMC8861500/ /pubmed/35211035 http://dx.doi.org/10.3389/fphys.2022.840995 Text en Copyright © 2022 Tzounakas, Anastasiadi, Lekka, Papageorgiou, Stamoulis, Papassideri, Kriebardis and Antonelou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Tzounakas, Vassilis L.
Anastasiadi, Alkmini T.
Lekka, Marilena E.
Papageorgiou, Effie G.
Stamoulis, Konstantinos
Papassideri, Issidora S.
Kriebardis, Anastasios G.
Antonelou, Marianna H.
Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title_full Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title_fullStr Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title_full_unstemmed Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title_short Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units
title_sort deciphering the relationship between free and vesicular hemoglobin in stored red blood cell units
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861500/
https://www.ncbi.nlm.nih.gov/pubmed/35211035
http://dx.doi.org/10.3389/fphys.2022.840995
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