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Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

Most of the protocols to analyze metabolic features of cell populations from different tissues rely on in vitro cell culture conditions. Here, we present a flow-cytometry-based protocol for measuring the respiratory chain function in permeabilized mouse microglia ex vivo. We describe microglial cell...

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Detalles Bibliográficos
Autores principales: Erny, Daniel, Dokalis, Nikolaos, Mezö, Charlotte, Mossad, Omar, Blank, Thomas, Prinz, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861812/
https://www.ncbi.nlm.nih.gov/pubmed/35243376
http://dx.doi.org/10.1016/j.xpro.2022.101186