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Mass cytometry staining for human bone marrow clinical samples

This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully...

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Detalles Bibliográficos
Autores principales: Hallisey, Margaret, Dennis, Jenna, Abrecht, Charlotte, Pistofidis, Romanos Sklavenitis, Schork, Abigail N., Lightbody, Elizabeth D., Heilpern-Mallory, Daniel, Severgnini, Mariano, Ghobrial, Irene M., Hodi, F. Stephen, Baginska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861824/
https://www.ncbi.nlm.nih.gov/pubmed/35243367
http://dx.doi.org/10.1016/j.xpro.2022.101163