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Mass cytometry staining for human bone marrow clinical samples
This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861824/ https://www.ncbi.nlm.nih.gov/pubmed/35243367 http://dx.doi.org/10.1016/j.xpro.2022.101163 |
| _version_ | 1784654946043428864 |
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| author | Hallisey, Margaret Dennis, Jenna Abrecht, Charlotte Pistofidis, Romanos Sklavenitis Schork, Abigail N. Lightbody, Elizabeth D. Heilpern-Mallory, Daniel Severgnini, Mariano Ghobrial, Irene M. Hodi, F. Stephen Baginska, Joanna |
| author_facet | Hallisey, Margaret Dennis, Jenna Abrecht, Charlotte Pistofidis, Romanos Sklavenitis Schork, Abigail N. Lightbody, Elizabeth D. Heilpern-Mallory, Daniel Severgnini, Mariano Ghobrial, Irene M. Hodi, F. Stephen Baginska, Joanna |
| author_sort | Hallisey, Margaret |
| collection | PubMed |
| description | This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples. |
| format | Online Article Text |
| id | pubmed-8861824 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-88618242022-03-02 Mass cytometry staining for human bone marrow clinical samples Hallisey, Margaret Dennis, Jenna Abrecht, Charlotte Pistofidis, Romanos Sklavenitis Schork, Abigail N. Lightbody, Elizabeth D. Heilpern-Mallory, Daniel Severgnini, Mariano Ghobrial, Irene M. Hodi, F. Stephen Baginska, Joanna STAR Protoc Protocol This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples. Elsevier 2022-02-17 /pmc/articles/PMC8861824/ /pubmed/35243367 http://dx.doi.org/10.1016/j.xpro.2022.101163 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Protocol Hallisey, Margaret Dennis, Jenna Abrecht, Charlotte Pistofidis, Romanos Sklavenitis Schork, Abigail N. Lightbody, Elizabeth D. Heilpern-Mallory, Daniel Severgnini, Mariano Ghobrial, Irene M. Hodi, F. Stephen Baginska, Joanna Mass cytometry staining for human bone marrow clinical samples |
| title | Mass cytometry staining for human bone marrow clinical samples |
| title_full | Mass cytometry staining for human bone marrow clinical samples |
| title_fullStr | Mass cytometry staining for human bone marrow clinical samples |
| title_full_unstemmed | Mass cytometry staining for human bone marrow clinical samples |
| title_short | Mass cytometry staining for human bone marrow clinical samples |
| title_sort | mass cytometry staining for human bone marrow clinical samples |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861824/ https://www.ncbi.nlm.nih.gov/pubmed/35243367 http://dx.doi.org/10.1016/j.xpro.2022.101163 |
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