Cargando…

Mass cytometry staining for human bone marrow clinical samples

This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hallisey, Margaret, Dennis, Jenna, Abrecht, Charlotte, Pistofidis, Romanos Sklavenitis, Schork, Abigail N., Lightbody, Elizabeth D., Heilpern-Mallory, Daniel, Severgnini, Mariano, Ghobrial, Irene M., Hodi, F. Stephen, Baginska, Joanna
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861824/ https://www.ncbi.nlm.nih.gov/pubmed/35243367 http://dx.doi.org/10.1016/j.xpro.2022.101163

Ejemplares similares

Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
por: Abrecht, Charlotte, et al.
Publicado: (2022)

Bone marrow niche in multiple myeloma and its precursor states
por: Sklavenitis-Pistofidis, Romanos, et al.
Publicado: (2019)

Multiple Myeloma With Amplification of Chr1q: Therapeutic Opportunity and Challenges
por: Sklavenitis-Pistofidis, Romanos, et al.
Publicado: (2022)

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples
por: Thrash, Emily M., et al.
Publicado: (2020)

Systematic characterization of therapeutic vulnerabilities in Multiple Myeloma with Amp1q reveals increased sensitivity to the combination of MCL1 and PI3K inhibitors
por: Sklavenitis-Pistofidis, Romanos, et al.
Publicado: (2023)

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells
por: Manuszak, Claire, et al.
Publicado: (2020)

Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry
por: Cunningham, Rachel A., et al.
Publicado: (2019)

Dysregulated Humoral and Cellular Response to Sars-Cov-2 Vaccination in Patients with MGUS and SMM
por: Konishi, Yoshinobu, et al.
Publicado: (2022)

Attenuated response to SARS-CoV-2 vaccine in patients with asymptomatic precursor stages of multiple myeloma and Waldenstrom macroglobulinemia
por: Konishi, Yoshinobu, et al.
Publicado: (2022)

Single cell characterization of myeloma and its precursor conditions reveals transcriptional signatures of early tumorigenesis
por: Boiarsky, Rebecca, et al.
Publicado: (2022)

P851: TARGETING GENE DEPENDENCIES IN MYC OVEREXPRESSING MULTIPLE MYELOMA
por: Hasan Bou Issa, L., et al.
Publicado: (2022)

Refractive index to evaluate staining specificity of extracellular vesicles by flow cytometry
por: de Rond, L., et al.
Publicado: (2019)

Profiling of circulating exosomal miRNAs in patients with Waldenström Macroglobulinemia
por: Bouyssou, Juliette M., et al.
Publicado: (2018)

A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining
por: Sonzogni-Desautels, Karine, et al.
Publicado: (2019)

Measurement of canine blood microparticles by flow cytometry: effect of anticoagulants and staining reagents
por: OZAWA, Tsuyoshi, et al.
Publicado: (2021)

Do more with Less: Improving High Parameter Cytometry Through Overnight Staining
por: Whyte, Carly E., et al.
Publicado: (2022)

Dual AO/EB Staining to Detect Apoptosis in Osteosarcoma Cells Compared with Flow Cytometry
por: Liu, Kuan, et al.
Publicado: (2015)

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry
por: Pirone, Daniele, et al.
Publicado: (2022)

Flow cytometry-based viability staining: an at-line tool for bioprocess monitoring of Sulfolobus acidocaldarius
por: Rastädter, Kerstin, et al.
Publicado: (2022)

High-throughput mass cytometry staining for deep phenotyping of human natural killer cells
por: Ben Amara, Amira, et al.
Publicado: (2022)

Pneumonitis resulting from radiation and immune checkpoint blockade illustrates characteristic clinical, radiologic and circulating biomarker features
por: Schoenfeld, Jonathan D., et al.
Publicado: (2019)

Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity
por: Hoving, Dennis, et al.
Publicado: (2023)

Intracellular Cytokine Staining and Flow Cytometry: Considerations for Application in Clinical Trials of Novel Tuberculosis Vaccines
por: Smith, Steven G., et al.
Publicado: (2015)

Comparison of two techniques for evaluation of pancreatic islet viability: flow cytometry and FDA/PI staining
por: Lemos, Natália Emerim, et al.
Publicado: (2015)

A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry
por: Qiu, Lin, et al.
Publicado: (2013)

Assessment of Gram- and Viability-Staining Methods for Quantifying Bacterial Community Dynamics Using Flow Cytometry
por: Duquenoy, Aurore, et al.
Publicado: (2020)

Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry
por: Channathodiyil, Prasanna, et al.
Publicado: (2021)

Phenotyping neuroblastoma cells through intelligent scrutiny of stain-free biomarkers in holographic flow cytometry
por: Pirone, Daniele, et al.
Publicado: (2023)

Immunophenotyping of Peripheral Blood and Bone Marrow Cells by Flow Cytometry
por: Akanni, EO, et al.
Publicado: (2006)

High-Throughput Isolation of Giant Viruses in Liquid Medium Using Automated Flow Cytometry and Fluorescence Staining
por: Khalil, Jacques Y. B., et al.
Publicado: (2016)

Lectin staining and flow cytometry reveals female-induced sperm acrosome reaction and surface carbohydrate reorganization
por: Kekäläinen, Jukka, et al.
Publicado: (2015)

Simple In-liquid Staining of Microbial Cells for Flow Cytometry Quantification of the Microbial Population in Marine Subseafloor Sediments
por: Mori, Fumiaki, et al.
Publicado: (2021)

Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions
por: Renner, Patrick, et al.
Publicado: (2022)

A Simplified, Sensitive Phagocytic Assay for Malaria Cultures Facilitated by Flow Cytometry of Differentially-Stained Cell Populations
por: Chan, Chuu Ling, et al.
Publicado: (2012)

Sequential staining of HIV gp140 to capture antigen-specific human B cells via flow cytometry
por: Townsley, Samantha M., et al.
Publicado: (2021)

A CONTRIBUTION TO STAINING METHODS : I. A DIFFERENTIAL STAIN FOR CONNECTIVE-TISSUE FIBRILLÆ AND RETICULUM. II. CHLORIDE OF IRON HÆMATOXYLIN FOR NUCLEI AND FIBRIN. III. PHOSPHOTUNGSTIC ACID HÆMATOXYLIN FOR NEUROGLIA FIBRES.
por: Mallory, F. B.
Publicado: (1900)

ON CERTAIN IMPROVEMENTS IN HISTOLOGICAL TECHNIQUE : I. A DIFFERENTIAL STAIN FOR AMŒBÆ COLI. II. PHOSPHOTUNGSTIC-ACID-HÆMATOXYLIN STAIN FOR CERTAIN TISSUE ELEMENTS. III. A METHOD OF FIXATION FOR NEUROGLIA FIBRES.
por: Mallory, F. B.
Publicado: (1897)

Isolation and Analysis of B-cell Progenitors from Bone Marrow by Flow Cytometry
por: Zhao, Hongchang, et al.
Publicado: (2023)

Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry
por: Keserue, Hans‐Anton, et al.
Publicado: (2012)

CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry
por: Nikiforova, Nadezhda, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_e07v17kj218aofljdr95fpjugq