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In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins

Many studies of the reconstitution of the Escherichia coli small ribosomal subunit from its individual molecular parts have been reported, but contrastingly, similar studies of the large ribosomal subunit have not been well performed to date. Here, we describe protocols for preparing the 33 ribosoma...

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Detalles Bibliográficos
Autores principales: Aoyama, Ryo, Masuda, Keiko, Shimojo, Masaru, Kanamori, Takashi, Ueda, Takuya, Shimizu, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863084/
https://www.ncbi.nlm.nih.gov/pubmed/34750629
http://dx.doi.org/10.1093/jb/mvab121