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Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat
KEY MESSAGE: We discovered a natural FT-A2 allele that increases grain number per spike in both pasta and bread wheat with limited effect on heading time. ABSTRACT: Increases in wheat grain yield are necessary to meet future global food demands. A previous study showed that loss-of-function mutation...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866389/ https://www.ncbi.nlm.nih.gov/pubmed/34825926 http://dx.doi.org/10.1007/s00122-021-03992-y |
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author | Glenn, Priscilla Zhang, Junli Brown-Guedira, Gina DeWitt, Noah Cook, Jason P. Li, Kun Akhunov, Eduard Dubcovsky, Jorge |
author_facet | Glenn, Priscilla Zhang, Junli Brown-Guedira, Gina DeWitt, Noah Cook, Jason P. Li, Kun Akhunov, Eduard Dubcovsky, Jorge |
author_sort | Glenn, Priscilla |
collection | PubMed |
description | KEY MESSAGE: We discovered a natural FT-A2 allele that increases grain number per spike in both pasta and bread wheat with limited effect on heading time. ABSTRACT: Increases in wheat grain yield are necessary to meet future global food demands. A previous study showed that loss-of-function mutations in FLOWERING LOCUS T2 (FT2) increase spikelet number per spike (SNS), an important grain yield component. However, these mutations were also associated with reduced fertility, offsetting the beneficial effect of the increases in SNS on grain number. Here, we report a natural mutation resulting in an aspartic acid to alanine change at position 10 (D10A) associated with significant increases in SNS and no negative effects on fertility. Using a high-density genetic map, we delimited the SNS candidate region to a 5.2-Mb region on chromosome 3AS including 28 genes. Among them, only FT-A2 showed a non-synonymous polymorphism (D10A) present in two different populations segregating for the SNS QTL on chromosome arm 3AS. These results, together with the known effect of the ft-A2 mutations on SNS, suggest that variation in FT-A2 is the most likely cause of the observed differences in SNS. We validated the positive effects of the A10 allele on SNS, grain number, and grain yield per spike in near-isogenic tetraploid wheat lines and in an hexaploid winter wheat population. The A10 allele is present at very low frequency in durum wheat and at much higher frequency in hexaploid wheat, particularly in winter and fall-planted spring varieties. These results suggest that the FT-A2 A10 allele may be particularly useful for improving grain yield in durum wheat and fall-planted common wheat varieties. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03992-y. |
format | Online Article Text |
id | pubmed-8866389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-88663892022-03-02 Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat Glenn, Priscilla Zhang, Junli Brown-Guedira, Gina DeWitt, Noah Cook, Jason P. Li, Kun Akhunov, Eduard Dubcovsky, Jorge Theor Appl Genet Original Article KEY MESSAGE: We discovered a natural FT-A2 allele that increases grain number per spike in both pasta and bread wheat with limited effect on heading time. ABSTRACT: Increases in wheat grain yield are necessary to meet future global food demands. A previous study showed that loss-of-function mutations in FLOWERING LOCUS T2 (FT2) increase spikelet number per spike (SNS), an important grain yield component. However, these mutations were also associated with reduced fertility, offsetting the beneficial effect of the increases in SNS on grain number. Here, we report a natural mutation resulting in an aspartic acid to alanine change at position 10 (D10A) associated with significant increases in SNS and no negative effects on fertility. Using a high-density genetic map, we delimited the SNS candidate region to a 5.2-Mb region on chromosome 3AS including 28 genes. Among them, only FT-A2 showed a non-synonymous polymorphism (D10A) present in two different populations segregating for the SNS QTL on chromosome arm 3AS. These results, together with the known effect of the ft-A2 mutations on SNS, suggest that variation in FT-A2 is the most likely cause of the observed differences in SNS. We validated the positive effects of the A10 allele on SNS, grain number, and grain yield per spike in near-isogenic tetraploid wheat lines and in an hexaploid winter wheat population. The A10 allele is present at very low frequency in durum wheat and at much higher frequency in hexaploid wheat, particularly in winter and fall-planted spring varieties. These results suggest that the FT-A2 A10 allele may be particularly useful for improving grain yield in durum wheat and fall-planted common wheat varieties. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03992-y. Springer Berlin Heidelberg 2021-11-26 2022 /pmc/articles/PMC8866389/ /pubmed/34825926 http://dx.doi.org/10.1007/s00122-021-03992-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Glenn, Priscilla Zhang, Junli Brown-Guedira, Gina DeWitt, Noah Cook, Jason P. Li, Kun Akhunov, Eduard Dubcovsky, Jorge Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title | Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title_full | Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title_fullStr | Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title_full_unstemmed | Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title_short | Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat |
title_sort | identification and characterization of a natural polymorphism in ft-a2 associated with increased number of grains per spike in wheat |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866389/ https://www.ncbi.nlm.nih.gov/pubmed/34825926 http://dx.doi.org/10.1007/s00122-021-03992-y |
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