Systematic Investigation of the Effects of Multiple SV40 Nuclear Localization Signal Fusion on the Genome Editing Activity of Purified SpCas9

The emergence of CRISPR-Cas9 technology has revolutionized both basic and translational biomedical research. For Cas9 nuclease to exert genome editing activity, nuclear localization signal (NLS) derived from simian virus 40 (SV40) T antigen is commonly installed as genetic fusion to direct the intra...

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Detalles Bibliográficos
Autores principales: Shui, Sailan, Wang, Shaojie, Liu, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869684/
https://www.ncbi.nlm.nih.gov/pubmed/35200436
http://dx.doi.org/10.3390/bioengineering9020083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869684/
https://www.ncbi.nlm.nih.gov/pubmed/35200436
http://dx.doi.org/10.3390/bioengineering9020083

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