Cargando…
PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage
PINK1 is a causative gene for Parkinson’s disease and the corresponding protein has been identified as a master regulator of mitophagy—the autophagic degradation of damaged mitochondria. It interacts with Beclin1 to regulate autophagy and initiate autophagosome formation, even outside the context of...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870463/ https://www.ncbi.nlm.nih.gov/pubmed/35203326 http://dx.doi.org/10.3390/cells11040678 |
_version_ | 1784656756975075328 |
---|---|
author | Brunelli, Francesco Torosantucci, Liliana Gelmetti, Vania Franzone, Davide Grünewald, Anne Krüger, Rejko Arena, Giuseppe Valente, Enza Maria |
author_facet | Brunelli, Francesco Torosantucci, Liliana Gelmetti, Vania Franzone, Davide Grünewald, Anne Krüger, Rejko Arena, Giuseppe Valente, Enza Maria |
author_sort | Brunelli, Francesco |
collection | PubMed |
description | PINK1 is a causative gene for Parkinson’s disease and the corresponding protein has been identified as a master regulator of mitophagy—the autophagic degradation of damaged mitochondria. It interacts with Beclin1 to regulate autophagy and initiate autophagosome formation, even outside the context of mitophagy. Several other pro-survival functions of this protein have been described and indicate that it might play a role in other disorders, such as cancer and proliferative diseases. In this study, we investigated a novel anti-apoptotic function of PINK1. To do so, we used SH-SY5Y neuroblastoma cells, a neuronal model used in Parkinson’s disease and cancer studies, to characterize the pro-survival functions of PINK1 in response to the apoptosis inducer staurosporine. In this setting, we found that staurosporine induces apoptosis but not mitophagy, and we demonstrated that PINK1 protects against staurosporine-induced apoptosis by impairing the pro-apoptotic cleavage of Beclin1. Our data also show that staurosporine-induced apoptosis is preceded by a phase of enhanced autophagy, and that PINK1 in this context regulates the switch from autophagy to apoptosis. PINK1 protein levels progressively decrease after treatment, inducing this switch. The PINK1–Beclin1 interaction is crucial in exerting this function, as mutants that are unable to interact do not show the anti-apoptotic effect. We characterized a new anti-apoptotic function of PINK1 that could provide options for treatment in proliferative or neurodegenerative diseases. |
format | Online Article Text |
id | pubmed-8870463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88704632022-02-25 PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage Brunelli, Francesco Torosantucci, Liliana Gelmetti, Vania Franzone, Davide Grünewald, Anne Krüger, Rejko Arena, Giuseppe Valente, Enza Maria Cells Article PINK1 is a causative gene for Parkinson’s disease and the corresponding protein has been identified as a master regulator of mitophagy—the autophagic degradation of damaged mitochondria. It interacts with Beclin1 to regulate autophagy and initiate autophagosome formation, even outside the context of mitophagy. Several other pro-survival functions of this protein have been described and indicate that it might play a role in other disorders, such as cancer and proliferative diseases. In this study, we investigated a novel anti-apoptotic function of PINK1. To do so, we used SH-SY5Y neuroblastoma cells, a neuronal model used in Parkinson’s disease and cancer studies, to characterize the pro-survival functions of PINK1 in response to the apoptosis inducer staurosporine. In this setting, we found that staurosporine induces apoptosis but not mitophagy, and we demonstrated that PINK1 protects against staurosporine-induced apoptosis by impairing the pro-apoptotic cleavage of Beclin1. Our data also show that staurosporine-induced apoptosis is preceded by a phase of enhanced autophagy, and that PINK1 in this context regulates the switch from autophagy to apoptosis. PINK1 protein levels progressively decrease after treatment, inducing this switch. The PINK1–Beclin1 interaction is crucial in exerting this function, as mutants that are unable to interact do not show the anti-apoptotic effect. We characterized a new anti-apoptotic function of PINK1 that could provide options for treatment in proliferative or neurodegenerative diseases. MDPI 2022-02-15 /pmc/articles/PMC8870463/ /pubmed/35203326 http://dx.doi.org/10.3390/cells11040678 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brunelli, Francesco Torosantucci, Liliana Gelmetti, Vania Franzone, Davide Grünewald, Anne Krüger, Rejko Arena, Giuseppe Valente, Enza Maria PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title | PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title_full | PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title_fullStr | PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title_full_unstemmed | PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title_short | PINK1 Protects against Staurosporine-Induced Apoptosis by Interacting with Beclin1 and Impairing Its Pro-Apoptotic Cleavage |
title_sort | pink1 protects against staurosporine-induced apoptosis by interacting with beclin1 and impairing its pro-apoptotic cleavage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870463/ https://www.ncbi.nlm.nih.gov/pubmed/35203326 http://dx.doi.org/10.3390/cells11040678 |
work_keys_str_mv | AT brunellifrancesco pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT torosantuccililiana pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT gelmettivania pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT franzonedavide pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT grunewaldanne pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT krugerrejko pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT arenagiuseppe pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage AT valenteenzamaria pink1protectsagainststaurosporineinducedapoptosisbyinteractingwithbeclin1andimpairingitsproapoptoticcleavage |