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Ranking the biases: The choice of OTUs vs. ASVs in 16S rRNA amplicon data analysis has stronger effects on diversity measures than rarefaction and OTU identity threshold

Advances in the analysis of amplicon sequence datasets have introduced a methodological shift in how research teams investigate microbial biodiversity, away from sequence identity-based clustering (producing Operational Taxonomic Units, OTUs) to denoising methods (producing amplicon sequence variant...

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Detalles Bibliográficos
Autores principales: Chiarello, Marlène, McCauley, Mark, Villéger, Sébastien, Jackson, Colin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870492/
https://www.ncbi.nlm.nih.gov/pubmed/35202411
http://dx.doi.org/10.1371/journal.pone.0264443