One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology

Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally. Reverse genetics is a valuable tool for the virological study and vaccine development for coronaviruses. Due to the large size and unsta...

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Detalles Bibliográficos
Autores principales: Zhou, Yanyang, Li, Chenxi, Ren, Cicheng, Hu, Jingbo, Song, Changxu, Wang, Xinjie, Li, Yanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870625/
https://www.ncbi.nlm.nih.gov/pubmed/35222326
http://dx.doi.org/10.3389/fmicb.2022.787739

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870625/
https://www.ncbi.nlm.nih.gov/pubmed/35222326
http://dx.doi.org/10.3389/fmicb.2022.787739

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