A Split NanoLuc Reporter Quantitatively Measures Circular RNA IRES Translation

Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation. While this assay is quantitative, it requires numerous controls and...

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Detalles Bibliográficos
Autores principales: Sehta, Priyanka, Wilhelm, Ann-Marie, Lin, Shu-Jun, Urman, Michelle A., MacNeil, Haley A., Fuchs, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871761/
https://www.ncbi.nlm.nih.gov/pubmed/35205400
http://dx.doi.org/10.3390/genes13020357

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871761/
https://www.ncbi.nlm.nih.gov/pubmed/35205400
http://dx.doi.org/10.3390/genes13020357

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