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A Split NanoLuc Reporter Quantitatively Measures Circular RNA IRES Translation
Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation. While this assay is quantitative, it requires numerous controls and...
Autores principales: | Sehta, Priyanka, Wilhelm, Ann-Marie, Lin, Shu-Jun, Urman, Michelle A., MacNeil, Haley A., Fuchs, Gabriele |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871761/ https://www.ncbi.nlm.nih.gov/pubmed/35205400 http://dx.doi.org/10.3390/genes13020357 |
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