Switching promotor recognition of phage RNA polymerase in silico along lab-directed evolution path

In this work, we computationally investigated how a viral RNA polymerase (RNAP) from bacteriophage T7 evolves into RNAP variants under lab-directed evolution to switch recognition from T7 promoter to T3 promoter in transcription initiation. We first constructed a closed initiation complex for the wi...

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Detalles Bibliográficos
Autores principales: E, Chao, Dai, Liqiang, Yu, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2022
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874028/
https://www.ncbi.nlm.nih.gov/pubmed/35031277
http://dx.doi.org/10.1016/j.bpj.2022.01.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874028/
https://www.ncbi.nlm.nih.gov/pubmed/35031277
http://dx.doi.org/10.1016/j.bpj.2022.01.007

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