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Switching promotor recognition of phage RNA polymerase in silico along lab-directed evolution path
In this work, we computationally investigated how a viral RNA polymerase (RNAP) from bacteriophage T7 evolves into RNAP variants under lab-directed evolution to switch recognition from T7 promoter to T3 promoter in transcription initiation. We first constructed a closed initiation complex for the wi...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Biophysical Society
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874028/ https://www.ncbi.nlm.nih.gov/pubmed/35031277 http://dx.doi.org/10.1016/j.bpj.2022.01.007 |