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Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1

The Chromatin Assembly Factor 1 is a heterotrimeric complex responsible for the nucleosome assembly during DNA replication and DNA repair. In humans, the largest subunit P150 is the major actor of this process. It has been recently considered as a tumor-associated protein due to its overexpression i...

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Autores principales: Ayoub, Joëlle, Buonanno, Martina, Di Fiore, Anna, De Simone, Giuseppina, Monti, Simona Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874411/
https://www.ncbi.nlm.nih.gov/pubmed/35216276
http://dx.doi.org/10.3390/ijms23042160
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author Ayoub, Joëlle
Buonanno, Martina
Di Fiore, Anna
De Simone, Giuseppina
Monti, Simona Maria
author_facet Ayoub, Joëlle
Buonanno, Martina
Di Fiore, Anna
De Simone, Giuseppina
Monti, Simona Maria
author_sort Ayoub, Joëlle
collection PubMed
description The Chromatin Assembly Factor 1 is a heterotrimeric complex responsible for the nucleosome assembly during DNA replication and DNA repair. In humans, the largest subunit P150 is the major actor of this process. It has been recently considered as a tumor-associated protein due to its overexpression in many malignancies. Structural and functional studies targeting P150 are still limited and only scarce information about this subunit is currently available. Literature data and bioinformatics analysis assisted the identification of a stable DNA binding domain, encompassing residues from 721 to 860 of P150 within the full-length protein. This domain was recombinantly produced and in vitro investigated. An acidic region modulating its DNA binding ability was also identified and characterized. Results showed similarities and differences between the P150 and its yeast homologue, namely Cac-1, suggesting that, although sharing a common biological function, the two proteins may also possess different features.
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spelling pubmed-88744112022-02-26 Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1 Ayoub, Joëlle Buonanno, Martina Di Fiore, Anna De Simone, Giuseppina Monti, Simona Maria Int J Mol Sci Article The Chromatin Assembly Factor 1 is a heterotrimeric complex responsible for the nucleosome assembly during DNA replication and DNA repair. In humans, the largest subunit P150 is the major actor of this process. It has been recently considered as a tumor-associated protein due to its overexpression in many malignancies. Structural and functional studies targeting P150 are still limited and only scarce information about this subunit is currently available. Literature data and bioinformatics analysis assisted the identification of a stable DNA binding domain, encompassing residues from 721 to 860 of P150 within the full-length protein. This domain was recombinantly produced and in vitro investigated. An acidic region modulating its DNA binding ability was also identified and characterized. Results showed similarities and differences between the P150 and its yeast homologue, namely Cac-1, suggesting that, although sharing a common biological function, the two proteins may also possess different features. MDPI 2022-02-15 /pmc/articles/PMC8874411/ /pubmed/35216276 http://dx.doi.org/10.3390/ijms23042160 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ayoub, Joëlle
Buonanno, Martina
Di Fiore, Anna
De Simone, Giuseppina
Monti, Simona Maria
Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title_full Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title_fullStr Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title_full_unstemmed Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title_short Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1
title_sort biochemical and structural insights into the winged helix domain of p150, the largest subunit of the chromatin assembly factor 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874411/
https://www.ncbi.nlm.nih.gov/pubmed/35216276
http://dx.doi.org/10.3390/ijms23042160
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