inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes

Cloning the genes and operons encoding heterologous functions in bacterial hosts is now almost exclusively carried out using plasmid vectors. This has multiple drawbacks, including the need for constant selection and variation in copy numbers. The chromosomal integration of transgenes has always off...

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Detalles Bibliográficos
Autores principales: Shukla, Ranti Dev, Zvara, Ágnes, Avramucz, Ákos, Biketova, Alona Yu., Nyerges, Akos, Puskás, László G., Fehér, Tamás
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875745/
https://www.ncbi.nlm.nih.gov/pubmed/35208691
http://dx.doi.org/10.3390/microorganisms10020236

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875745/
https://www.ncbi.nlm.nih.gov/pubmed/35208691
http://dx.doi.org/10.3390/microorganisms10020236

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