The ATP/Mg(2+) Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome

Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg(2+), and DTT. ATP and Mg(2+) are included to maintain 26S proteasome functionality. However, most cellular proteasom...

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Detalles Bibliográficos
Autores principales: Morozov, Alexey, Astakhova, Tatyana, Erokhov, Pavel, Karpov, Vadim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875927/
https://www.ncbi.nlm.nih.gov/pubmed/35200531
http://dx.doi.org/10.3390/mps5010015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875927/
https://www.ncbi.nlm.nih.gov/pubmed/35200531
http://dx.doi.org/10.3390/mps5010015

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