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The ATP/Mg(2+) Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome
Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg(2+), and DTT. ATP and Mg(2+) are included to maintain 26S proteasome functionality. However, most cellular proteasom...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875927/ https://www.ncbi.nlm.nih.gov/pubmed/35200531 http://dx.doi.org/10.3390/mps5010015 |