Cargando…

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyan...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jiao, Cao, Ke, Wang, Lirong, Dong, Wenxuan, Zhang, Xiao, Liu, Weisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879194/
https://www.ncbi.nlm.nih.gov/pubmed/35214840
http://dx.doi.org/10.3390/plants11040507
_version_ 1784658841026166784
author Wang, Jiao
Cao, Ke
Wang, Lirong
Dong, Wenxuan
Zhang, Xiao
Liu, Weisheng
author_facet Wang, Jiao
Cao, Ke
Wang, Lirong
Dong, Wenxuan
Zhang, Xiao
Liu, Weisheng
author_sort Wang, Jiao
collection PubMed
description Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyanin biosynthesis in peach fruit is unknown. In order to understand this induction mechanism, comprehensive transcriptome and metabolome were performed in fruit flesh treated with four different sugars for 12 and 24 h, respectively. Here, we found that cyanidin-3-O-(6-O-p-coumaroyl) glucosides accumulated in fruit flesh treated with glucose, sucrose, sorbitol, and fructose in vitro. Two key structural genes of the anthocyanin biosynthesis pathway, namely, PpDFR and PpUFGT, were upregulated in the flesh of sugar-treated peach fruit. By contrast, the two main transcription factors (TFs) PpMYB10.1 and PpBL regulating anthocyanin biosynthetic genes in peach fruit were not upregulated accordingly. Interestingly, two MYB family genes (PpMYB6 and PpMYB44-like) and three bHLH family genes (PpbHLH35, PpbHLH51, and PpbHLH36-like) were upregulated. A dual luciferase assay revealed that PpMYB6 strongly activated the PpUFGT promoter when it was co-infiltrated with PpbHLH35, PpbHLH51, and PpbHLH36-like. When PpMYB44-like was co-infiltrated with PpbHLH35, it also potently activated the PpUFGT promoter. The results of this study help clarify the molecular mechanisms by which glucose, sucrose, sorbitol, and fructose regulate anthocyanin accumulation in peach fruit.
format Online
Article
Text
id pubmed-8879194
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88791942022-02-26 Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro Wang, Jiao Cao, Ke Wang, Lirong Dong, Wenxuan Zhang, Xiao Liu, Weisheng Plants (Basel) Article Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyanin biosynthesis in peach fruit is unknown. In order to understand this induction mechanism, comprehensive transcriptome and metabolome were performed in fruit flesh treated with four different sugars for 12 and 24 h, respectively. Here, we found that cyanidin-3-O-(6-O-p-coumaroyl) glucosides accumulated in fruit flesh treated with glucose, sucrose, sorbitol, and fructose in vitro. Two key structural genes of the anthocyanin biosynthesis pathway, namely, PpDFR and PpUFGT, were upregulated in the flesh of sugar-treated peach fruit. By contrast, the two main transcription factors (TFs) PpMYB10.1 and PpBL regulating anthocyanin biosynthetic genes in peach fruit were not upregulated accordingly. Interestingly, two MYB family genes (PpMYB6 and PpMYB44-like) and three bHLH family genes (PpbHLH35, PpbHLH51, and PpbHLH36-like) were upregulated. A dual luciferase assay revealed that PpMYB6 strongly activated the PpUFGT promoter when it was co-infiltrated with PpbHLH35, PpbHLH51, and PpbHLH36-like. When PpMYB44-like was co-infiltrated with PpbHLH35, it also potently activated the PpUFGT promoter. The results of this study help clarify the molecular mechanisms by which glucose, sucrose, sorbitol, and fructose regulate anthocyanin accumulation in peach fruit. MDPI 2022-02-13 /pmc/articles/PMC8879194/ /pubmed/35214840 http://dx.doi.org/10.3390/plants11040507 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jiao
Cao, Ke
Wang, Lirong
Dong, Wenxuan
Zhang, Xiao
Liu, Weisheng
Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title_full Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title_fullStr Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title_full_unstemmed Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title_short Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro
title_sort two myb and three bhlh family genes participate in anthocyanin accumulation in the flesh of peach fruit treated with glucose, sucrose, sorbitol, and fructose in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879194/
https://www.ncbi.nlm.nih.gov/pubmed/35214840
http://dx.doi.org/10.3390/plants11040507
work_keys_str_mv AT wangjiao twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro
AT caoke twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro
AT wanglirong twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro
AT dongwenxuan twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro
AT zhangxiao twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro
AT liuweisheng twomybandthreebhlhfamilygenesparticipateinanthocyaninaccumulationinthefleshofpeachfruittreatedwithglucosesucrosesorbitolandfructoseinvitro