Effect of Aphidicolin, a Reversible Inhibitor of Eukaryotic Nuclear DNA Replication, on the Production of Genetically Modified Porcine Embryos by CRISPR/Cas9

Mosaicism is the most important limitation for one-step gene editing in embryos by CRISPR/Cas9 because cuts and repairs sometimes take place after the first DNA replication of the zygote. To try to minimize the risk of mosaicism, in this study a reversible DNA replication inhibitor was used after th...

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Detalles Bibliográficos
Autores principales: Navarro-Serna, Sergio, Piñeiro-Silva, Celia, Luongo, Chiara, Parrington, John, Romar, Raquel, Gadea, Joaquín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880323/
https://www.ncbi.nlm.nih.gov/pubmed/35216252
http://dx.doi.org/10.3390/ijms23042135

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880323/
https://www.ncbi.nlm.nih.gov/pubmed/35216252
http://dx.doi.org/10.3390/ijms23042135

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