Cargando…
Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens
Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine, and the inactivated vaccine is used to prevent and control SVA infection. To develop a new chromatography strategy for the purification and concentration of SVA vaccine antigens, we inserted a 6×His-tag at the VP1 C-termin...
Autores principales: | Fan, Junhao, Xiao, Peiyu, Kong, Dongni, Liu, Xinran, Meng, Liang, An, Tongqing, Cai, Xuehui, Wang, Haiwei, Yu, Li |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880742/ https://www.ncbi.nlm.nih.gov/pubmed/35214628 http://dx.doi.org/10.3390/vaccines10020170 |
Ejemplares similares
-
Magnetic One-Step Purification of His-Tagged Protein
by Bare Iron Oxide Nanoparticles
por: Schwaminger, Sebastian P., et al.
Publicado: (2019) -
One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles
por: Zhou, Li‐Jian, et al.
Publicado: (2021) -
A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli
por: Ederth, Josefine, et al.
Publicado: (2009) -
The Stem-Loop I of Senecavirus A IRES Is Essential for Cap-Independent Translation Activity and Virus Recovery
por: Wang, Nana, et al.
Publicado: (2021) -
Senecavirus A Entry Into Host Cells Is Dependent on the Cholesterol-Mediated Endocytic Pathway
por: Jia, Meiyu, et al.
Publicado: (2022)