Engineering miRNA features into siRNAs: Guide-strand bulges are compatible with gene repression

Synthetic siRNA guide strands are typically designed with perfect complementarity to the passenger strand and the target mRNA. We examined whether siRNAs with intentional guide-strand bulges are functional in vitro and in vivo. Importantly, this was done by systematic shortening of the passenger str...

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Detalles Bibliográficos
Autores principales: Hauptmann, Judith, Hehne, Vivien, Balzer, Melanie, Bethge, Lucas, Wikstrom Lindholm, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881630/
https://www.ncbi.nlm.nih.gov/pubmed/35251767
http://dx.doi.org/10.1016/j.omtn.2022.02.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881630/
https://www.ncbi.nlm.nih.gov/pubmed/35251767
http://dx.doi.org/10.1016/j.omtn.2022.02.004

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