High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM(+) cells

Lymphocytes in the mouse small intestine (SI) epithelium are critical to establish effective barrier immunity. Here, we describe a magnetic cell separation protocol that employs anti-epithelial cellular adhesion molecule (EpCAM) antibodies to deplete epithelial cells and to enrich for intraepithelia...

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Detalles Bibliográficos
Autores principales: Prakhar, Praveen, Gonzalez, Victoria, Park, Jung-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885746/
https://www.ncbi.nlm.nih.gov/pubmed/35243383
http://dx.doi.org/10.1016/j.xpro.2022.101207

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885746/
https://www.ncbi.nlm.nih.gov/pubmed/35243383
http://dx.doi.org/10.1016/j.xpro.2022.101207

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