An optimized protocol to assess SUMOylation in the plant Capsella rubella using two-component DEX-inducible transformants

Here, we present an efficient protocol to test the SUMOylation of a target protein in the plant Capsella rubella based on overexpression of dexamethasone (DEX)-inducible tagged proteins. We describe the construction of two-component, FLAG-tagged DEX-inducible plasmids. We then detail the transformat...

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Detalles Bibliográficos
Autores principales: Dong, Yang, Hu, Zhi-Cheng, Østergaard, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885766/
https://www.ncbi.nlm.nih.gov/pubmed/35243380
http://dx.doi.org/10.1016/j.xpro.2022.101197

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885766/
https://www.ncbi.nlm.nih.gov/pubmed/35243380
http://dx.doi.org/10.1016/j.xpro.2022.101197

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