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Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses

The SARS-CoV-2 B.1.1.529 lineage, Omicron variant, was first detected in November 2021 and carries 32 amino acid mutations in the spike protein (15 in RBD) and exhibits significant escape of neutralizing antibodies targeting the parental SARS-CoV-2 virus. Here, we performed a high-resolution multipl...

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Autores principales: Wang, Lin-Fa, Tan, Chee Wah, Chia, Wan Ni, Zhu, Feng, Young, Barnaby, Chantasrisawad, Napaporn, Hwa, Shi-Hsia, Yeoh, Aileen Ying-Yan, Lim, Beng Lee, Yap, Wee Chee, Pada, Surinder Kaur, Tan, Seow Yen, Jantarabenjakul, Watsamon, Chen, Shiwei, Zhang, Jinyan, Mah, Yun Yan, Chen, Vivian, Chen, Mark, Wacharapluesadee, Supaporn, Putcharoen, Opass, Lye, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887082/
https://www.ncbi.nlm.nih.gov/pubmed/35233568
http://dx.doi.org/10.21203/rs.3.rs-1362541/v1
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author Wang, Lin-Fa
Tan, Chee Wah
Chia, Wan Ni
Zhu, Feng
Young, Barnaby
Chantasrisawad, Napaporn
Hwa, Shi-Hsia
Yeoh, Aileen Ying-Yan
Lim, Beng Lee
Yap, Wee Chee
Pada, Surinder Kaur
Tan, Seow Yen
Jantarabenjakul, Watsamon
Chen, Shiwei
Zhang, Jinyan
Mah, Yun Yan
Chen, Vivian
Chen, Mark
Wacharapluesadee, Supaporn
Putcharoen, Opass
Lye, David
author_facet Wang, Lin-Fa
Tan, Chee Wah
Chia, Wan Ni
Zhu, Feng
Young, Barnaby
Chantasrisawad, Napaporn
Hwa, Shi-Hsia
Yeoh, Aileen Ying-Yan
Lim, Beng Lee
Yap, Wee Chee
Pada, Surinder Kaur
Tan, Seow Yen
Jantarabenjakul, Watsamon
Chen, Shiwei
Zhang, Jinyan
Mah, Yun Yan
Chen, Vivian
Chen, Mark
Wacharapluesadee, Supaporn
Putcharoen, Opass
Lye, David
author_sort Wang, Lin-Fa
collection PubMed
description The SARS-CoV-2 B.1.1.529 lineage, Omicron variant, was first detected in November 2021 and carries 32 amino acid mutations in the spike protein (15 in RBD) and exhibits significant escape of neutralizing antibodies targeting the parental SARS-CoV-2 virus. Here, we performed a high-resolution multiplex (16-plex) surrogate virus neutralization assay covering all major SARS-CoV-2 variants and pre-emergent ACE2-binding sarbecoviruses against 20 different human serum panels from infected, vaccinated and hybrid immune individuals which had vaccine-breakthrough infections or infection followed by vaccination. Among all sarbecoviruses tested, we observed 1.1 to 4.7-, 2.3 to 10.3- and 0.7 to 33.3-fold reduction in neutralization activities to SARS-CoV-2 Beta, Omicron and SARS-CoV-1, respectively. Among the SARS-CoV-2 related sarbecoviruses, it is found that the genetically more distant bat RaTG13 and pangolin GX-P5L sarbecoviruses had less neutralization escape than Omicron. Our data suggest that the SARS-CoV-2 variants emerged from the changed immune landscape of human populations are more potent in escaping neutralizing antibodies, from infection or vaccination, than pre-emergent sarbecoviruses naturally evolved in animal populations with no or less immune selection pressure.
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spelling pubmed-88870822022-03-02 Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses Wang, Lin-Fa Tan, Chee Wah Chia, Wan Ni Zhu, Feng Young, Barnaby Chantasrisawad, Napaporn Hwa, Shi-Hsia Yeoh, Aileen Ying-Yan Lim, Beng Lee Yap, Wee Chee Pada, Surinder Kaur Tan, Seow Yen Jantarabenjakul, Watsamon Chen, Shiwei Zhang, Jinyan Mah, Yun Yan Chen, Vivian Chen, Mark Wacharapluesadee, Supaporn Putcharoen, Opass Lye, David Res Sq Article The SARS-CoV-2 B.1.1.529 lineage, Omicron variant, was first detected in November 2021 and carries 32 amino acid mutations in the spike protein (15 in RBD) and exhibits significant escape of neutralizing antibodies targeting the parental SARS-CoV-2 virus. Here, we performed a high-resolution multiplex (16-plex) surrogate virus neutralization assay covering all major SARS-CoV-2 variants and pre-emergent ACE2-binding sarbecoviruses against 20 different human serum panels from infected, vaccinated and hybrid immune individuals which had vaccine-breakthrough infections or infection followed by vaccination. Among all sarbecoviruses tested, we observed 1.1 to 4.7-, 2.3 to 10.3- and 0.7 to 33.3-fold reduction in neutralization activities to SARS-CoV-2 Beta, Omicron and SARS-CoV-1, respectively. Among the SARS-CoV-2 related sarbecoviruses, it is found that the genetically more distant bat RaTG13 and pangolin GX-P5L sarbecoviruses had less neutralization escape than Omicron. Our data suggest that the SARS-CoV-2 variants emerged from the changed immune landscape of human populations are more potent in escaping neutralizing antibodies, from infection or vaccination, than pre-emergent sarbecoviruses naturally evolved in animal populations with no or less immune selection pressure. American Journal Experts 2022-02-23 /pmc/articles/PMC8887082/ /pubmed/35233568 http://dx.doi.org/10.21203/rs.3.rs-1362541/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Wang, Lin-Fa
Tan, Chee Wah
Chia, Wan Ni
Zhu, Feng
Young, Barnaby
Chantasrisawad, Napaporn
Hwa, Shi-Hsia
Yeoh, Aileen Ying-Yan
Lim, Beng Lee
Yap, Wee Chee
Pada, Surinder Kaur
Tan, Seow Yen
Jantarabenjakul, Watsamon
Chen, Shiwei
Zhang, Jinyan
Mah, Yun Yan
Chen, Vivian
Chen, Mark
Wacharapluesadee, Supaporn
Putcharoen, Opass
Lye, David
Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title_full Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title_fullStr Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title_full_unstemmed Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title_short Differential escape of neutralizing antibodies by SARS-CoV-2 Omicron and pre-emergent sarbecoviruses
title_sort differential escape of neutralizing antibodies by sars-cov-2 omicron and pre-emergent sarbecoviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887082/
https://www.ncbi.nlm.nih.gov/pubmed/35233568
http://dx.doi.org/10.21203/rs.3.rs-1362541/v1
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