Cargando…

Reconstitution of 3′ end processing of mammalian pre-mRNA reveals a central role of RBBP6

The 3′ ends of almost all eukaryotic mRNAs are generated in an essential two-step processing reaction: endonucleolytic cleavage of an extended precursor followed by the addition of a poly(A) tail. By reconstituting the reaction from overproduced and purified proteins, we provide a minimal list of 14...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schmidt, Moritz, Kluge, Florian, Sandmeir, Felix, Kühn, Uwe, Schäfer, Peter, Tüting, Christian, Ihling, Christian, Conti, Elena, Wahle, Elmar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2022
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887130/ https://www.ncbi.nlm.nih.gov/pubmed/35177537 http://dx.doi.org/10.1101/gad.349217.121

Ejemplares similares

Reconstitution of mammalian cleavage factor II involved in 3′ processing of mRNA precursors
por: Schäfer, Peter, et al.
Publicado: (2018)

Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33
por: Schönemann, Lars, et al.
Publicado: (2014)

RBBP6 activates the pre-mRNA 3′ end processing machinery in humans
por: Boreikaite, Vytaute, et al.
Publicado: (2022)

The nuclear poly(A) binding protein of mammals, but not of fission yeast, participates in mRNA polyadenylation
por: Kühn, Uwe, et al.
Publicado: (2017)

Establishment of 5′–3′ interactions in mRNA independent of a continuous ribose-phosphate backbone
por: Kluge, Florian, et al.
Publicado: (2020)

RNA binding proteins Smaug and Cup induce CCR4–NOT-dependent deadenylation of the nanos mRNA in a reconstituted system
por: Pekovic, Filip, et al.
Publicado: (2023)

Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch
por: Götze, Michael, et al.
Publicado: (2017)

Structural analysis of 70S ribosomes by cross-linking/mass spectrometry reveals conformational plasticity
por: Tüting, Christian, et al.
Publicado: (2020)

Human pre-mRNA 3′ end processing: reconstituting is believing
por: Yoon, Yoseop, et al.
Publicado: (2022)

Hypoxia-induced RBBP7 promotes esophagus cancer progression by inducing CDK4 expression: RBBP7 promotes esophagus cancer progression
por: Wang, Renfeng, et al.
Publicado: (2022)

DWNN, a novel ubiquitin-like domain, implicates RBBP6 in mRNA processing and ubiquitin-like pathways
por: Pugh, David JR, et al.
Publicado: (2006)

Prognosis value of RBBP8 expression in plasma cell myeloma
por: Zhang, Weilong, et al.
Publicado: (2019)

Rbbp4 Suppresses Premature Differentiation of Embryonic Stem Cells
por: Huang, Yikai, et al.
Publicado: (2021)

RBBP4 Enhances Platinum Chemo Resistance in Lung Adenocarcinoma
por: Wang, Nianwu, et al.
Publicado: (2021)

Resolution and Reconstitution of a Mammalian Membrane
por: Racker, Efraim
Publicado: (1969)

Reconstituting the Mammalian Apoptotic Switch in Yeast
por: Polčic, Peter, et al.
Publicado: (2020)

Deadenylation of mRNA by the CCR4–NOT complex in Drosophila: molecular and developmental aspects
por: Temme, Claudia, et al.
Publicado: (2014)

Prognostic value of CtIP/RBBP8 expression in breast cancer
por: Soria-Bretones, Isabel, et al.
Publicado: (2013)

Simple methods for the 3′ biotinylation of RNA
por: Moritz, Bodo, et al.
Publicado: (2014)

RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma
por: Yu, Ning, et al.
Publicado: (2018)

X-linked RBBP7 mutation causes maturation arrest and testicular tumors
por: Li, Jingping, et al.
Publicado: (2023)

Structural assessment of the full-length wild-type tumor suppressor protein p53 by mass spectrometry-guided computational modeling
por: Di Ianni, Alessio, et al.
Publicado: (2023)

Reconstitution of mammalian mitochondrial translation system capable of correct initiation and long polypeptide synthesis from leaderless mRNA
por: Lee, Muhoon, et al.
Publicado: (2020)

GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression
por: Peter, Daniel, et al.
Publicado: (2017)

Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases
por: Lu, Fei, et al.
Publicado: (2016)

Investigating the Effects of RBBP6 Gene Expression on Telomerase Activity in Cervical Cancer Cells
por: Mosweu, Mpho, et al.
Publicado: (2020)

The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex
por: Han, Jianming, et al.
Publicado: (2019)

Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy
por: Wang, Yicun, et al.
Publicado: (2022)

Expression and clinical significance of RBBP4 gene in lower-grade glioma: An integrative analysis
por: Liang, Ruofei, et al.
Publicado: (2023)

Mechanism of RBBP8‐mediated homologous recombination repair in gastric cancer synthetic lethal
por: Yu, Yang, et al.
Publicado: (2023)

Mammalian Germ Cell Development: From Mechanism to In Vitro Reconstitution
por: Saitou, Mitinori
Publicado: (2021)

RBBP6: a potential biomarker of apoptosis induction in human cervical cancer cell lines
por: Moela, Pontsho, et al.
Publicado: (2016)

RBBP6 increases radioresistance and serves as a therapeutic target for preoperative radiotherapy in colorectal cancer
por: Xiao, Chao, et al.
Publicado: (2018)

RBBP6 Is Abundantly Expressed in Human Cervical Carcinoma and May Be Implicated in Its Malignant Progression
por: Dlamini, Zodwa, et al.
Publicado: (2019)

WDR5, ASH2L, and RBBP5 control the efficiency of FOS transcript processing
por: Teoh, Peik Lin, et al.
Publicado: (2014)

Unveiling the molecular structure and role of RBBP4/7: implications for epigenetic regulation and cancer research
por: Cai, Lize, et al.
Publicado: (2023)

The Y3** ncRNA promotes the 3′ end processing of histone mRNAs
por: Köhn, Marcel, et al.
Publicado: (2015)

Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy
por: Chang, Chunmei, et al.
Publicado: (2021)

High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate
por: Freibaum, Brian D., et al.
Publicado: (2021)

Oligoadenylation of 3′ decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells
por: Harnisch, Christiane, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_on6s9o15166h8rhdih7o8a7e0d