Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics

In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-in...

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Detalles Bibliográficos
Autores principales: Salek, Mogjiborahman, Förster, Jonas D., Lehmann, Wolf-Dieter, Riemer, Angelika B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888373/
https://www.ncbi.nlm.nih.gov/pubmed/35119480
http://dx.doi.org/10.1007/s00216-022-03931-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888373/
https://www.ncbi.nlm.nih.gov/pubmed/35119480
http://dx.doi.org/10.1007/s00216-022-03931-w

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