Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics

In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-in...

Descripción completa

Detalles Bibliográficos
Autores principales: Salek, Mogjiborahman, Förster, Jonas D., Lehmann, Wolf-Dieter, Riemer, Angelika B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888373/
https://www.ncbi.nlm.nih.gov/pubmed/35119480
http://dx.doi.org/10.1007/s00216-022-03931-w
_version_ 1784661131707547648
author Salek, Mogjiborahman
Förster, Jonas D.
Lehmann, Wolf-Dieter
Riemer, Angelika B.
author_facet Salek, Mogjiborahman
Förster, Jonas D.
Lehmann, Wolf-Dieter
Riemer, Angelika B.
author_sort Salek, Mogjiborahman
collection PubMed
description In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-internal standards potentially compromises the detection and quantitation especially of low abundant cellular peptides. Therefore, it is important to adopt guidelines to prevent false-positive identifications of endogenous light peptides as well as errors in their quantitation from biological samples. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-03931-w.
format Online
Article
Text
id pubmed-8888373
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-88883732022-03-02 Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics Salek, Mogjiborahman Förster, Jonas D. Lehmann, Wolf-Dieter Riemer, Angelika B. Anal Bioanal Chem Communication In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-internal standards potentially compromises the detection and quantitation especially of low abundant cellular peptides. Therefore, it is important to adopt guidelines to prevent false-positive identifications of endogenous light peptides as well as errors in their quantitation from biological samples. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-03931-w. Springer Berlin Heidelberg 2022-02-04 2022 /pmc/articles/PMC8888373/ /pubmed/35119480 http://dx.doi.org/10.1007/s00216-022-03931-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Communication
Salek, Mogjiborahman
Förster, Jonas D.
Lehmann, Wolf-Dieter
Riemer, Angelika B.
Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title_full Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title_fullStr Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title_full_unstemmed Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title_short Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
title_sort light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888373/
https://www.ncbi.nlm.nih.gov/pubmed/35119480
http://dx.doi.org/10.1007/s00216-022-03931-w
work_keys_str_mv AT salekmogjiborahman lightcontaminationinstableisotopelabelledinternalpeptidestandardsisfrequentandapotentialsourceoffalsediscoveryandquantitationerrorinproteomics
AT forsterjonasd lightcontaminationinstableisotopelabelledinternalpeptidestandardsisfrequentandapotentialsourceoffalsediscoveryandquantitationerrorinproteomics
AT lehmannwolfdieter lightcontaminationinstableisotopelabelledinternalpeptidestandardsisfrequentandapotentialsourceoffalsediscoveryandquantitationerrorinproteomics
AT riemerangelikab lightcontaminationinstableisotopelabelledinternalpeptidestandardsisfrequentandapotentialsourceoffalsediscoveryandquantitationerrorinproteomics

Ejemplares similares