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Site-directed fluorescence approaches to monitor the structural dynamics of proteins using intrinsic Trp and labeled with extrinsic fluorophores

Comprehensive understanding of a protein’s function depends on having reliable, sophisticated tools to study protein structural dynamics in physiologically-relevant conditions. Here, we present an effective, robust step-by-step protocol to monitor the structural dynamics (including hydration dynamic...

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Detalles Bibliográficos
Autores principales: Brahma, Rupasree, Das, Anindita, Raghuraman, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889417/
https://www.ncbi.nlm.nih.gov/pubmed/35252885
http://dx.doi.org/10.1016/j.xpro.2022.101200